|
|
Over the years, the team led by Bento Soares (University of Iowa) has optimized methods for producing cDNA libraries, a technically challenging undertaking, and is continuing to produce libraries of the highest quality. Individual clones from these libraries have been arrayed at Livermore and distributed worldwide for characterization by the international I.M.A.G.E. (Integrated Molecular Analysis of Gene Expression) Consortium. To date, over 3 million I.M.A.G.E. clone replicas have been sent to more than 1000 laboratories worldwide; end users analyze the clones and return data on them (http://www.ncbi.nlm.nih.gov/dbEST/index.html).
At the Santa Fe meeting, Soares described further progress in developing cDNA libraries and serial subtractive hybridization strategies (within and across different pooled libraries). These strategies are expected to minimize redundancy and identify cDNAs not yet represented in publicly available collections of human, mouse, and rat cDNAs.
Soares observed that finding novel cDNAs is increasingly challenging as researchers approach completion of human and mouse gene-discovery efforts. His group uses pools of I.M.A.G.E. clones, from which ESTs have been derived, as drivers in hybridizations with single or multiple normalized libraries, thus generating subtracted libraries enriched for new cDNAs. Sequence analysis of two subtracted libraries indicated a fourfold reduction in representation of the driver clones.
Charles Auffray (Centre National de la Recherche Scientifique) reviewed the goals of EURO-IMAGE, which include generating and sequencing a master set of unique full-length cDNA clones (based on I.M.A.G.E. Consortium resources), representing 3000 transcripts and 6 Mb of finished sequence. The European consortium is integrating its efforts with those in the United States and Japan. A meeting of I.M.A.G.E. participants is tentatively planned for March 1999 in Kazusa, Japan.
Auffray outlined some major challenges of systematic large-scale efforts to obtain the human "transcriptome" (the complete collection of all unique sequenced gene transcripts). He also discussed generating insights into gene function by exploring similarities and linking the information to the proteome |
| Author: Aaron Hall |
| |
Author Bio:
|
|
 |
|
| |
Related Articles |
| |
Don't Graduate - What Students Should Know About College, But No One Ever Tells Them
|
| |
How to Schedule a Book Signing
|
| |
IT Certification Exams: 3 Most Popular Mistakes
|
| |
Atomic Clocks: Why They Are The Best
|
| |
Making Biodiesel at Home
|
| |
"Classroom Management, the Dance of the Dolphin" author Mark Kennedy: BOOK REVIEW
|
| |
Immerse Yourself in the Greek Language and Culture
|
| |
Holistic Approach May Be The Key to Curing Addiction
|
| |
Celine Dion Biography
|
| |
Portable Crowd and Protestor Control Systems
|
| |
|
|
|
